Personal Information

Hobbies: Swimming, Triangle Chromatography Discussion Group

Favorite Place in Chapel Hill:

Undergraduate Institution

Development and Characterization of Colloidal Silica Coated Superficially Porous Particles

Several types of particles and columns have been developed for use with high pressure liquid chromatography (HPLC) with hopes of producing optimal separation efficiency and sample loadability. Open tubular columns and those packed with pellicular, superficially porous, non-porous and completely porous particles have been investigated. The majority of current development in HPLC is focused on totally porous particles with ever decreasing diameter. The use of these particles leads to increased efficiency and decreased run times while maintaining good sample loadability. The difficulty with smaller particles, however, is that pressures above the values typically used for HPLC are needed. With the advent of ultra-high pressure liquid chromatography (UHPLC), the higher pressures required by the use of smaller particles can now be supplied. While the higher pressures are helpful, there is still a need to more efficiently separate macromolecules. Recent studies have indicated that both superficially porous particles and open tubular columns may be particularly useful in this regard.